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KMID : 0613820220320090690
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Journal of Life Science
2022 Volume.32 No. 9 p.690 ~ p.697
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Analysis of Microsatellite Patterns in the Genome of Red Sea Cucumber
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Lee Tae-Wook
Kim Sam-Woong
Kim Jung-Sun
Chi Won-Jae
Bang Woo-Young
Kim Jang-Hyeon
Yang Chul-Woong
Bang Kyu-Ho
Gal Sang-Wan
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Abstract
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This study was conducted to analyze genome of red sea cucumber and to use it as basic data for the development of genetic markers for red sea cucumber. Microsatellite marker analysis of Ulleungdo_ normal and Ulleungdo_native red sea cucumbers revealed that dinucleotide simple sequence repeats (SSRs) had the highest ratio, at 81.3～81.4%, and the number of the detected SSRs tended to decrease as the number of repeating sequence units in SSRs increased. In general, microsatellites with between 5 and 10 iterations were most common. As the size of the SSR repeating sequence units increased, the SSR iterations gradually decreased. The di-, tri-, and tetra-nucleotides in SSRs were detected in the highest numbers as (AT)5, (AAT)5, and (AAAT)5, respectively. (CG) and (CCG) had very low frequencies compared to the numbers of other repeating SSR units. The numbers of di-and tri-nucleotide repeats were up to 35 and 32, respectively, and then increased discontinuously up to 44 and 43 repeats, respectively. Tetra-, penta-, and hexa-nucleotides in SSRs occurred in numbers up to 25, 21 and 14, respectively. This analysis of red sea cucumber indicated that it maintains its own repetition sequence and repetition number; therefore, we suggest that using it as basic data for molecular marker will be possible in future research.
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KEYWORD
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Genomics, microsatellite, red sea cucumber, repeat number, repeat unit
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